Chemical Synthesis of Oligonucleotides
نویسندگان
چکیده
It can fairly be said that synthetic oligonucleotides are the fuel that drives the engine of molecular biology. Nearly every technique in use today in molecular biology employs chemically synthesized DNAs or RNAs. This includes PCR, Real-Time PCR, DNA sequencing, site directed mutagenesis, single-nucleotide polymorphism (SNP) assays, microarrays, and the rapidly expanding world of small RNAs. Unlike other reagents, however, oligonucleotides are not stock items that can be stored, warehouse fashion, until needed. Each oligonucleotide is custom made according to the specific needs of the individual researcher and purified according to the application for which it is intended.
منابع مشابه
Specific Inhibition of the Expression of the Promyelocytic Leukemia (PML) Protein by Anti-Sense Oligonucleotides
In the present study, using anti-sense oligonucleotides the inhibition of expression of the PML protein hasbeen investigated. The anti-sense oligonucleotides were designed against the translation initiation site ofthe PML gene, and their effects were investigated on cellular growth and DNA synthesis. Incubation of normalhuman fibroblast cells with the anti-sense oligonucleotid...
متن کاملSyringe method for stepwise chemical synthesis of oligonucleotides.
A simple procedure is described for synthesis of oligonucleotides by phosphate chemistry. Chains can be constructed rapidly with minimal equipment (a syringe and reagent bottles). The method is illustrated by synthesis of d-TGCAGGTT. Pertinent supporting data on the effect of variations in the detritylation, condensation, oxidation, capping and cleavage steps in the synthetic approach and in is...
متن کاملSynthesis and biophysical analysis of modified thymine-containing DNA oligonucleotides.
We report the synthesis of a 5-formyl-2'-deoxyuridine (5fU) phosphoramidite and the preparation of oligonucleotides comprising all known, naturally observed eukaryotic thymidine modifications. Biophysical characterization of the synthetic oligonucleotides indicates that 5fU, but not the other T-derivatives, can alter DNA structures.
متن کاملA Biotechnological Perspective on The Affinity Magnetic Separation and Purification Based on Oligonucleotides
The rapidly growing field of biotechnology has created a critical need for simple, fast andhigh-throughput processes for the separation and purification of biomolecules from biologicalmatrices. In recent years, several bioseparation techniques have been proposed as advancedalternatives to the classical separation methods. These modern processes emphasize ultrahighselective and sensitive analysi...
متن کاملChemical synthesis of oligonucleotides using acetone as a washing solvent.
Acetone is being proposed as the main washing solvent during automated synthesis of oligonucleotides to reduce acetonitrile usage in the assembly process and endure the impact of global acetonitrile shortage.
متن کامل